Ionic basis of methacholine-induced shrinkage of dissociated eccrine clear cells

  title={Ionic basis of methacholine-induced shrinkage of dissociated eccrine clear cells},
  author={Yuichi Suzuki and Minoru Ohtsuyama and G. Samman and Fusako Sato and Kenzo Sato},
  journal={The Journal of Membrane Biology},
The goal of the present study was to elucidate the ionic mechanisms by which cholinergic stimulation induces cell shrinkage in eccrine clear cells. Dissociated Rhesus monkey eccrine sweat clear cells were prepared by collagenase digestion of freshly isolated secretory coils and immobilized on a glass slide in a perfusion chamber at 30°C. The cell was visualized by light microscopy with differential interference contract (DIC) and was recorded with a video system (15,000× total magnification… CONTINUE READING
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