Ionic and nucleotide requirements for microtubule polymerization in vitro.

@article{Olmsted1975IonicAN,
  title={Ionic and nucleotide requirements for microtubule polymerization in vitro.},
  author={Joanna B. Olmsted and Gary G. Borisy},
  journal={Biochemistry},
  year={1975},
  volume={14 13},
  pages={2996-3005}
}
The ionic and nucleotide requirements for the in vitro polymerization of microtubules from purified brain tubulin have been characterized by viscometry. Protein was purified by successive cycles of a temperature dependent assembly-diassembly scheme. Maximal polymerization occurred at a concentration of 0.1 M Pipes (piperazine-N,N'-bis(2-ethanesulfonic acid)); increasing ionic strength by addition of NaCl to samples prepared in lower buffer concentrations did not result in an equivalent level of… CONTINUE READING