Ion trap tandem mass spectrometry applied to small multiply charged oligonucleotides with a modified base.

@article{McLuckey1994IonTT,
  title={Ion trap tandem mass spectrometry applied to small multiply charged oligonucleotides with a modified base.},
  author={Scott A McLuckey and S Habibi-Goudarzi},
  journal={Journal of the American Society for Mass Spectrometry},
  year={1994},
  volume={5 8},
  pages={740-7}
}
Two isomeric oligodeoxynucleotide hexamers, 5'-d(N-6meATGCAT)-3' and 5'-d(ATGSmeCAT)-3', were subjected to analysis by electrospray and ion trap mass spectrometry. In the case of the isomer with a modified adenine, location of the modified base in the sequence was straightforward and a triple mass spectrometry experiment provided information on the identity of the modification. In contrast, the isomer with the methylated cytosine did not yield definitive information on the location or identity… CONTINUE READING