Ion transport by primary cultures of canine tracheal epithelium: Methodology, morphology, and electrophysiology


Canine tracheal epithelial cells were isolated by enzymatic and mechanical dispersion and cultured on permeable supports. The cells formed confluent monolayers and retained most of the morphologic characteristics of the intact epithelium, including apical microvilli, apical tight junctions, and a moderately interdigitated lateral intercellular space. The… (More)
DOI: 10.1007/BF01868429


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