Ion selectivity reversal and induction of voltage-gating by site-directed mutations in the Paracoccus denitrificans porin.

@article{Saxena1999IonSR,
  title={Ion selectivity reversal and induction of voltage-gating by site-directed mutations in the Paracoccus denitrificans porin.},
  author={Krishna G Saxena and Viktoria Drosou and Elke Maier and Roland Benz and B. Ludwig},
  journal={Biochemistry},
  year={1999},
  volume={38 7},
  pages={2206-12}
}
The porin from Paracoccus denitrificans, a slightly anion specific outer membrane pore protein, was expressed in Escherichia coli, isolated from inclusion bodies, and refolded in the presence of urea and detergents. The purified recombinant protein was reconstituted into black lipid bilayer membranes and showed no difference in its functional properties in comparison to the native porin isolated from P.denitrificans membranes. To investigate the molecular basis of its ion selectivity and… CONTINUE READING

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