Ion-pair reversed-phase high-performance liquid chromatography analysis of oligonucleotides: retention prediction.

@article{Gilar2002IonpairRH,
  title={Ion-pair reversed-phase high-performance liquid chromatography analysis of oligonucleotides: retention prediction.},
  author={M Bernal Gilar and Kenneth J. Fountain and Yeva Budman and Uwe Dieter Neue and Kurt R Yardley and Paul D. Rainville and Reb J. Russell and John C. Gebler},
  journal={Journal of chromatography. A},
  year={2002},
  volume={958 1-2},
  pages={167-82}
}
An ion-pair reversed-phase HPLC method was evaluated for the separation of synthetic oligonucleotides. Mass transfer in the stationary phase was found to be a major factor contributing to peak broadening on porous C18 stationary phases. A small sorbent particle size (2.5 microm), elevated temperature and a relatively slow flow-rate were utilized to enhance mass transfer. A short 50 mm column allows for an efficient separation up to 30mer oligonucleotides. The separation strategy consists of a… CONTINUE READING
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