Ion-exclusion controlled size-exclusion chromatography of methacrylic acid-methyl methacrylate copolymers.

@article{Porsch2000IonexclusionCS,
  title={Ion-exclusion controlled size-exclusion chromatography of methacrylic acid-methyl methacrylate copolymers.},
  author={Bedřich Porsch and I Hillang and Anders M. Karlsson and L O Sundel{\"o}f},
  journal={Journal of chromatography. A},
  year={2000},
  volume={872 1-2},
  pages={91-9}
}
Controlled ion-exclusion allows compensation of hydrophobic adsorption in size-exclusion chromatography of negatively charged methacrylic acid-methyl methacrylate (Eudragit) polymers using methanol as a mobile phase. Properly selected low-ionic-strength conditions below 5 mM LiCl provide correct separation in the size-exclusion mode. Possible disturbing effects, mainly related to light scattering, under low-salt conditions are discussed and shown to be negligible if on-line concentration-light… CONTINUE READING