Ion-exchange membrane chromatography method for rapid and efficient purification of recombinant baculovirus and baculovirus gp64 protein.

@article{Wu2007IonexchangeMC,
  title={Ion-exchange membrane chromatography method for rapid and efficient purification of recombinant baculovirus and baculovirus gp64 protein.},
  author={Chunxiao Wu and Ker Yin Soh and Shu De Wang},
  journal={Human gene therapy},
  year={2007},
  volume={18 7},
  pages={665-72}
}
Significant progress in the application of baculoviral vectors for gene delivery into mammalian cells calls for the development of powerful methods for virus purification and concentration. We report here a novel and efficient method based on membrane chromatography to prepare baculoviral stocks. On a strong cation-exchange membrane unit, baculovirus in insect cell culture supernatants was captured at a flow rate of 3 ml/min and efficiently eluted at the same flow rate with a physiological… CONTINUE READING

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