Ion-exchange column chromatographic method for assaying purine metabolic pathway enzymes.

@article{Ward1998IonexchangeCC,
  title={Ion-exchange column chromatographic method for assaying purine metabolic pathway enzymes.},
  author={Herbert B. Ward and Donald Baldwin and T Wang and Harold Warner and Keith Seymour and Ch. Marquardt and Edward O. McFalls and John E. Foker},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1998},
  volume={707 1-2},
  pages={295-300}
}
High energy phosphate levels fall rapidly during cardiac ischemia and recover slowly (more than one week) during reperfusion. The slow recovery of ATP may reflect a lack of purine metabolic precursors and/or increased activity of purine catabolic enzymes such as 5'-nucleotidase (5'-NT, EC 3.1.3.5) and adenosine deaminase (ADA, EC 3.5.4.4). The activity of enzymes involved in both the catabolism of ATP precursors (5-NT and ADA) and the restoration of ATP from slow synthetic pathways [adenosine… CONTINUE READING