Ion dependence of the Bacillus subtilis RNase P reaction.

@article{Gardiner1985IonDO,
  title={Ion dependence of the Bacillus subtilis RNase P reaction.},
  author={Ken Gardiner and Terry L. Marsh and Norman R. Pace},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 9},
  pages={5415-9}
}
The properties of the Bacillus subtilis RNase P are characterized with regard to the types and concentrations of monovalent and divalent ions required to potentiate precursor tRNA cleavage by the protein-RNA holoenzyme and the catalytic RNA alone. The ionic dependence of the RNase P RNA-catalyzed reaction in part seems due to a requirement for ion shielding between substrate and catalytic RNAs. The RNase P protein, which binds to RNA nonspecifically and tightly, likely serves, in part, as a… CONTINUE READING