Ion channels in microbes.

@article{Martinac2008IonCI,
  title={Ion channels in microbes.},
  author={Boris Martinac and Yoshiro Saimi and Ching Kung},
  journal={Physiological reviews},
  year={2008},
  volume={88 4},
  pages={
          1449-90
        }
}
Studies of ion channels have for long been dominated by the animalcentric, if not anthropocentric, view of physiology. The structures and activities of ion channels had, however, evolved long before the appearance of complex multicellular organisms on earth. The diversity of ion channels existing in cellular membranes of prokaryotes is a good example. Although at first it may appear as a paradox that most of what we know about the structure of eukaryotic ion channels is based on the structure… 

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References

SHOWING 1-10 OF 454 REFERENCES

Mechanosensitive Channels in Prokaryotes

  • B. Martinac
  • Biology
    Cellular Physiology and Biochemistry
  • 2001
TLDR
This review summarizes an area of the MS channel research where major progress has occurred and presents examples of the recent promising developments that may ultimately lead to the understanding of the biophysical principles and evolutionary origins of mechanosensory transduction.

Evolutionary origins of mechanosensitive ion channels.

The Role of Bacterial Channels in Cell Physiology

TLDR
The proposed role of mechanosensitive (MS) channels is the rapid and nonspecific release of solutes from the cell in response to the generation of excessive turgor pressure and this model has now been tested and verified for E. coli.

A biological role for prokaryotic ClC chloride channels

TLDR
It is shown that Escherichia coli uses chloride channels of the widespread ClC family in the extreme acid resistance response and proposed that the channels function as an electrical shunt for an outwardly directed virtual proton pump that is linked to amino acid decarboxylation.

Mechanosensitive channels in archaea

TLDR
The phylogenetic analysis revealed that prokaryotic MS channels of large and small conductance originated from a common ancestral molecule resembling the bacterial MscL channel protein, indicating the existence of a common gating mechanism for bacterial as well as archaeal MS channels according to the bilayer model.

Bacterial ion channels and their eukaryotic homologues

TLDR
This review describes prokaryotic channels whose structures have been determined and presents amino‐acid sequences of eukaryotic proteins related in their primary sequences to bacterial mechanosensitive channels.

Regulation of cytoplasmic pH in bacteria.

  • I. Booth
  • Biology
    Microbiological reviews
  • 1985
TLDR
The aim of this review of pH homeostasis in bacteria is to assess the role of transport systems in the regulation of cytoplasmic pH and to consider the principal questions which remain to be answered.

TOK Homologue in Neurospora crassa: First Cloning and Functional Characterization of an Ion Channel in a Filamentous Fungus

TLDR
NcTOKA single-channel activity was characterized by rapid flickering between the open and closed states with a unitary conductance of 16 pS and was effectively blocked by extracellular Ca2+, verapamil, quinine, and TEA+ but was insensitive to Cs+, 4-aminopyridine, and glibenclamide.
...