Involvement of the carboxyl terminus of the third intracellular loop of the cannabinoid CB1 receptor in constitutive activation of Gs.

@article{Abadji1999InvolvementOT,
  title={Involvement of the carboxyl terminus of the third intracellular loop of the cannabinoid CB1 receptor in constitutive activation of Gs.},
  author={V Abadji and Jean Lucas-Lenard and Cindy N Chin and Debra A. Kendall},
  journal={Journal of neurochemistry},
  year={1999},
  volume={72 5},
  pages={2032-8}
}
The human cannabinoid receptor CB1 functionally couples primarily to Gi-, but also to Gs-mediated pathways to modulate intracellular cyclic AMP (cAMP) levels. To probe the features of the receptor that may be involved in promoting interactions with one G protein type over another, we generated the L341A/A342L mutant CB1 receptor. The double mutation involved the swap in position of two adjacent residues in the carboxyl-terminal segment of the third intracellular loop of CB1. This resulted in… CONTINUE READING