Involvement of the carboxyl-terminal region of the alpha 1 subunit in voltage-dependent inactivation of cardiac calcium channels.

@article{Klckner1995InvolvementOT,
  title={Involvement of the carboxyl-terminal region of the alpha 1 subunit in voltage-dependent inactivation of cardiac calcium channels.},
  author={Udo Kl{\"o}ckner and Gabor Mikala and M{\'a}ria V{\'a}radi and Gyula Varadi and Arnold Schwartz},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 29},
  pages={17306-10}
}
Intracellular application of proteases increases cardiac calcium current to a level similar to beta-adrenergic stimulation. Using transiently transfected HEK 293 cells, we studied the molecular mechanism underlying calcium channel stimulation by proteolytic treatment. Perfusion of HEK cells, coexpressing the human cardiac (hHT) alpha 1, alpha 2, and beta 3 subunits, with 1 mg/ml of trypsin or carboxypeptidase A, increased the peak amplitude of the calcium channel current 3-4-fold without… CONTINUE READING