Involvement of the High-Affinity Receptor for IgG (FcyRI; CD64) in Enhanced Tumor Cell Cytotoxicity of Neutrophils During Granulocyte Colony-Stimulating Factor Therapy

Abstract

Three different classes of Fc receptors for IgG (FcyR) are currently distinguished in humans, of which polymorphonuclear phagocytes (PMN) normally express both low-affinity receptor classes-FcyR11 (CD32) and FcyRlll (CDI 6). During therapy with granulocyte colony-stimulating factor (G-CSF), neutrophils from patients with various malignancies and different hematologic disorders werevfound to additionally express high levels of the receptor with high affinity for IgG (FcyRI; CD64). For these patients, the relative fluorescence intensity (rFI) for FcyRl was 5.3 (range, 1.7 to 10.3; n = 19). compared with 1 .O (range, 1 .O to 1.1 ; n = 8) for healthy donors. The expression of FcyRl during GCSF therapy could be confirmed by using a panel of six CD64-specific antibodies, and by showing mRNA for FcyRI. So far, three genes for FcyRl have been identified, encoding four distinct transcription products. By reverse transcriptase-polymerase chain reaction technology, tran-

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Cite this paper

@inproceedings{Valerius2003InvolvementOT, title={Involvement of the High-Affinity Receptor for IgG (FcyRI; CD64) in Enhanced Tumor Cell Cytotoxicity of Neutrophils During Granulocyte Colony-Stimulating Factor Therapy}, author={Thomas Valerius and Roland Repp and Susanne Berthold and E. Platzer and Joachim Richard Kalden and Martin Gramatzki}, year={2003} }