Involvement of the Arg179 in the active site of human IL-6.

  title={Involvement of the Arg179 in the active site of human IL-6.},
  author={Valerie Fontaine and Rocco Savino and Rosaria Arcone and Laurence Th de Wit and Jan P. G. Brakenhoff and J. Content and G. Ciliberto},
  journal={European journal of biochemistry},
  volume={211 3},
Three internal-amino acid deletions of amino acids 171-179 of human interleukin 6 (IL-6) were introduced at the cDNA level. While all deletion proteins were biologically inactive, immunoprecipitations with a set of conformation-specific anti-(IL-6) monoclonal antibodies showed that only mutant delta 177-179 does not present major alterations in folding. This finding, together with the observation that delta 177-179 is not able to compete with IL-6 for binding to the soluble human IL-6 receptor… CONTINUE READING

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