Involvement of phosphoinositide 3-kinase in insulin- or IGF-1-induced membrane ruffling.

@article{Kotani1994InvolvementOP,
  title={Involvement of phosphoinositide 3-kinase in insulin- or IGF-1-induced membrane ruffling.},
  author={Kazuaki Kotani and Kazuyoshi Yonezawa and Kenta Hara and Hiroshi Ueda and Yoshiaki Kitamura and Hiroshi Sakaue and Akikazu Ando and A Chavanieu and Bernard Calas and F Grigorescu},
  journal={The EMBO journal},
  year={1994},
  volume={13 10},
  pages={2313-21}
}
Insulin, IGF-1 or EGF induce membrane ruffling through their respective tyrosine kinase receptors. To elucidate the molecular link between receptor activation and membrane ruffling, we microinjected phosphorylated peptides containing YMXM motifs or a mutant 85 kDa subunit of phosphoinositide (PI) 3-kinase (delta p85) which lacks a binding site for the catalytic 110 kDa subunit of PI 3-kinase into the cytoplasm of human epidermoid carcinoma KB cells. Both inhibited the association of insulin… CONTINUE READING