Involvement of highly sulfated chondroitin sulfate in the metastasis of the Lewis lung carcinoma cells.

Abstract

The altered expression of cell surface chondroitin sulfate (CS) and dermatan sulfate (DS) in cancer cells has been demonstrated to play a key role in malignant transformation and tumor metastasis. However, the functional highly sulfated structures in CS/DS chains and their involvement in the process have not been well documented. In the present study, a structural analysis of CS/DS from two mouse Lewis lung carcinoma (3LL)-derived cell lines with different metastatic potentials revealed a higher proportion of Delta(4,5)HexUA-GalNAc(4,6-O-disulfate) generated from E-units (GlcUA-GalNAc(4, 6-O-disulfate)) in highly metastatic LM66-H11 cells than in low metastatic P29 cells, although much less CS/DS is expressed by LM66-H11 than P29 cells. This key finding prompted us to study the role of CS-E-like structures in experimental lung metastasis. The metastasis of LM66-H11 cells to lungs was effectively inhibited by enzymatic removal of tumor cell surface CS or by preadministration of CS-E rich in E-units in a dose-dependent manner. In addition, immunocytochemical analysis showed that LM66-H11 rather than P29 cells expressed more strongly the CS-E epitope, which was specifically recognized by the phage display antibody GD3G7. More importantly, this antibody and a CS-E decasaccharide fraction, the minimal structure recognized by GD3G7, strongly inhibited the metastasis of LM66-H11 cells probably by modifying the proliferative and invading behavior of the metastatic tumor cells. These results suggest that the E-unit-containing epitopes are involved in the metastatic process and a potential target for the diagnosis and treatment of malignant tumors.

DOI: 10.1074/jbc.M806015200

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@article{Li2008InvolvementOH, title={Involvement of highly sulfated chondroitin sulfate in the metastasis of the Lewis lung carcinoma cells.}, author={Fuchuan Li and Gerdy B. ten Dam and S Thirusenthil Murugan and Shuhei Yamada and Taishi Hashiguchi and Shuji Mizumoto and Kayoko Oguri and Minoru Okayama and Toin H. van Kuppevelt and Kazuyuki Sugahara}, journal={The Journal of biological chemistry}, year={2008}, volume={283 49}, pages={34294-304} }