Involvement of fixLJ in the regulation of nitrogen fixation in Azorhizobium caulinodans.

@article{Kaminski1991InvolvementOF,
  title={Involvement of fixLJ in the regulation of nitrogen fixation in Azorhizobium caulinodans.},
  author={Pierre Alexandre Kaminski and Claudine Elmerich},
  journal={Molecular microbiology},
  year={1991},
  volume={5 3},
  pages={
          665-73
        }
}
A gene bank of Azorhizobium caulinodans DNA constructed in the bacteriophage lambda GEM11 was screened with Rhizobium meliloti fixL and fixJ genes as probes. One positive recombinant phage, ORS lambda L, was isolated. The nucleotide sequence of a 3.7 kb fragment was established. Two open reading frames of 1512bp and 613bp were identified as fixL and fixJ. Kanamycin cartridges were inserted into the cloned fixL and fixJ genes and recombined into the host genome. The resulting mutants were Nif… CONTINUE READING

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