Involvement of cis and trans Golgi apparatus elements in the intracellular sorting and targeting of acid hydrolases to lysosomes.

@article{Minnifield1986InvolvementOC,
  title={Involvement of cis and trans Golgi apparatus elements in the intracellular sorting and targeting of acid hydrolases to lysosomes.},
  author={N M Minnifield and Kim E. Creek and Pl{\'a}cido Navas and Dorothy M. Morr{\'e}},
  journal={European journal of cell biology},
  year={1986},
  volume={42 1},
  pages={92-100}
}
To delineate the traffic route through the Golgi apparatus followed by newly synthesized lysosomal enzymes, we subfractionated the Golgi apparatus of rat liver by preparative free-flow electrophoresis into cisternae fractions of increasing content of trans face markers and decreasing contents of markers for the cis face. NADPase was used to mark median… CONTINUE READING