Involvement of chitinases of Bacillus thuringiensis during pathogenesis in insects.

@article{Sampson1998InvolvementOC,
  title={Involvement of chitinases of Bacillus thuringiensis during pathogenesis in insects.},
  author={Mark N Sampson and Graham W. Gooday},
  journal={Microbiology},
  year={1998},
  volume={144 ( Pt 8)},
  pages={2189-94}
}
Bacillus thuringiensis subsp. israelensis IPS78 and B. thuringiensis subsp. aizawai HD133 both secreted exochitinase activity when grown in a medium containing chitin. Allosamidin, a specific chitinase inhibitor, inhibited activity from both strains, with IC50 values of about 50 microM with colloidal chitin as substrate and between 1 and 10 microM with 4-methylumbelliferyl-diacetylchitobioside and 4-methylumbelliferyl-triacetylchitotrioside as substrates. The involvement of these chitinolytic… CONTINUE READING