Involvement of carbohydrates as multiple low affinity interaction sites in the self-association of the aggregation factor from the marine sponge Microciona prolifera.

@article{Misevic1987InvolvementOC,
  title={Involvement of carbohydrates as multiple low affinity interaction sites in the self-association of the aggregation factor from the marine sponge Microciona prolifera.},
  author={Gradimir N. Misevic and Jukka Finne and Max M. Burger},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 12},
  pages={5870-7}
}
Cell aggregation in the marine sponge Microciona prolifera is mediated by a multimillion molecular weight aggregation factor (MAF) and is based on two functional properties, a Ca2+-independent cell binding activity and a Ca2+-dependent factor-factor self-interaction. Monoclonal antibodies were prepared against purified MAF, and one clone was characterized which selectively inhibited the MAF-MAF association activity. Binding of the blocking antibody (Block 1) to MAF demonstrated that this… CONTINUE READING

Connections & Topics

Mentioned Connections BETA
Such blocking antibodies precipitated a small molecular weight protein - free glycan fraction prepared from MAF by Pronase digestion , thus indicating that the highly repetitive epitope is located in the carbohydrate portion of the molecule .
Such blocking antibodies precipitated a small molecular weight protein - free glycan fraction prepared from MAF by Pronase digestion , thus indicating that the highly repetitive epitope is located in the carbohydrate portion of the molecule .
Such blocking antibodies precipitated a small molecular weight protein - free glycan fraction prepared from MAF by Pronase digestion , thus indicating that the highly repetitive epitope is located in the carbohydrate portion of the molecule .
All Topics