Involvement of a gene on chromosome 9 in human fibroblast Interferon production

@article{Meager1979InvolvementOA,
  title={Involvement of a gene on chromosome 9 in human fibroblast Interferon production},
  author={Anthony Meager and H E Graves and Derek C. Burke and Dallas M. Swallow},
  journal={Nature},
  year={1979},
  volume={280},
  pages={493-495}
}
HUMAN fibroblast interferon is a single monomeric gly-coprotein1, and presumably a single structural gene codes for the human interferon polypeptide. It was originally reported that chromosomes 2 and 5 were necessary together for human interferon production2. More recent evidence suggests that both chromosomes 2 and 5 carry separate human interferon genes3,4. However, we have found, in two separate series of human–mouse somatic cell hybrid cell lines and their diphtheria toxin-resistant… 
Human immune interferon gene is located on chromosome 12
A cDNA clone for human immune interferon (IFN-gamma) gene sequences, plasmid p69, was used to chromosomally map the IFN-gamma gene by detecting human IFN-gamma gene sequences in DNA isolated from
SOMATIC CELL GENETIC ANALYSIS OF INTERFERON PRODUCTION AND RESPONSE *
TLDR
Recent work with somatic cell hybrids is discussed to further elucidate the genetic control behind interferon systhesis and response and to discuss the roles played by each of these chromosomes.
Human beta interferon gene localization and expression in somatic cell hybrids
The human fibroblast interferon gene beta 1 was mapped to human chromosome 9. Sequence homology with a beta 1 cDNA clone was detected in both genomic DNA and induced mRNA of human/mouse or
Interferon-beta-related DNA is dispersed in the human genome.
TLDR
The finding that DNA related to theIFN-beta 1 gene (and IFN-alpha 1 gene) is dispersed in the human genome raises new questions about the origins of the interferon genes.
Clustering of leukocyte and fibroblast interferon genes of human chromosome 9.
TLDR
Gene mapping was accomplished by blot hybridization of cloned interferon complementary DNA to DNA from human-mouse cell hybrids with a translocation involving human chromosome 9.
Heterogeneity of poly(I).poly(C)-induced human fibroblast interferon mRNA species
TLDR
It is shown here, using gel electrophoresis of mRNA, that two populations of translationally active human fibroblast IFN mRNA molecules exist—an abundant ‘14 S’ species and a scarce ‘11 S” species.
Mouse interferon alpha and beta genes are linked at the centromere proximal region of chromosome 4.
TLDR
Southern blot analysis of DNA from mouse/hamster hybrids compared with the analysis of chromosome markers showed that both the MuIFN-alpha and the MuifN-beta genes are located on chromosome 4.
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TLDR
It is postulated that there are at least two asyntenic gene loci for the expression of interferon in human cells and that the presence of both chromosomes 2 and 5 was necessary forinterferon production.
Genetic analysis of the cell surface: association of human chromosome 5 with sensitivity to diphtheria toxin in mouse-human somatic cell hybrids.
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TLDR
It is shown that the sensitivity of the hybrid cells is due to a gene or genes located on human chromosome 5, and mouse-human cell hybrids in which chromosome 5 is present are as sensitive to the toxin as human cells, which hybrids without chromosome 5 are as resistant as mouse cells.
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A human-mouse hybrid, DUR 4 (Solomon et al., 1976), containing a human X/15 translocation chromosome and also chromosome 5, among other human chromosomes, was used in a double selection system to
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TLDR
Human genes coding for hypoxanthine phosphoribosyltransferase, HPRT, G6PD, glucose-6-phosphate dehydrogenase, and phosphoglycerate kinase have been assigned to specific regions on the long arm of the X chromosome by somatic cell gentic techniques and evidence suggests a gene order on the X longArm of centromere-PGK-HPRT-G6PD.
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TLDR
Oxidation of this polypeptide with periodic acid and subsequent staining with Fuchsin base indicates that it contains carbohydrate ans suggests that the human fibroblast interferon is a glycoprotein.
Induction of Interferon in Chick Cells by Temperature-sensitive Mutants of Sindbis Virus
TLDR
It is postulated that interferon induction is dependent on a low threshold level of RNA synthesis occurring early in infection as well as on the input multiplicity of the virus.
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TLDR
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