Involvement of a gene on chromosome 9 in human fibroblast Interferon production

@article{Meager1979InvolvementOA,
  title={Involvement of a gene on chromosome 9 in human fibroblast Interferon production},
  author={Anthony Meager and Hazel E. Graves and Derek C. Burke and Dallas M. Swallow},
  journal={Nature},
  year={1979},
  volume={280},
  pages={493-495}
}
HUMAN fibroblast interferon is a single monomeric gly-coprotein1, and presumably a single structural gene codes for the human interferon polypeptide. It was originally reported that chromosomes 2 and 5 were necessary together for human interferon production2. More recent evidence suggests that both chromosomes 2 and 5 carry separate human interferon genes3,4. However, we have found, in two separate series of human–mouse somatic cell hybrid cell lines and their diphtheria toxin-resistant… 

Human immune interferon gene is located on chromosome 12

A cDNA clone for human immune interferon (IFN-gamma) gene sequences, plasmid p69, was used to chromosomally map the IFN-gamma gene by detecting human IFN-gamma gene sequences in DNA isolated from

SOMATIC CELL GENETIC ANALYSIS OF INTERFERON PRODUCTION AND RESPONSE *

Recent work with somatic cell hybrids is discussed to further elucidate the genetic control behind interferon systhesis and response and to discuss the roles played by each of these chromosomes.

Human I8 Interferon Gene Localization and Expression in Somatic Cell Hybrids

Interferon mRNA that did not share sequence homology with the P,l cDNA clone was detected in lines containing human chromosomes 2 and 5 but lacking chromosome 9, suggesting the presence of other unlinked interferon sequences in the human genome.

Human beta interferon gene localization and expression in somatic cell hybrids

Interferon mRNA that did not share sequence homology with the beta 1 cDNA clone was detected in lines containing human chromosomes 2 and 5 but lacking chromosome 9, suggesting the presence of other unlinked interferon sequences in the human genome.

Interferon-beta-related DNA is dispersed in the human genome.

The finding that DNA related to theIFN-beta 1 gene (and IFN-alpha 1 gene) is dispersed in the human genome raises new questions about the origins of the interferon genes.

Clustering of leukocyte and fibroblast interferon genes of human chromosome 9.

Gene mapping was accomplished by blot hybridization of cloned interferon complementary DNA to DNA from human-mouse cell hybrids with a translocation involving human chromosome 9.

Heterogeneity of poly(I).poly(C)-induced human fibroblast interferon mRNA species

It is shown here, using gel electrophoresis of mRNA, that two populations of translationally active human fibroblast IFN mRNA molecules exist—an abundant ‘14 S’ species and a scarce ‘11 S” species.

References

SHOWING 1-10 OF 13 REFERENCES

The somatic cell genetics of human interferon: assignment of human interferon loci to chromosomes 2 and 5.

It is postulated that there are at least two asyntenic gene loci for the expression of interferon in human cells and that the presence of both chromosomes 2 and 5 was necessary forinterferon production.

Genetic analysis of the cell surface: association of human chromosome 5 with sensitivity to diphtheria toxin in mouse-human somatic cell hybrids.

It is shown that the sensitivity of the hybrid cells is due to a gene or genes located on human chromosome 5, and mouse-human cell hybrids in which chromosome 5 is present are as sensitive to the toxin as human cells, which hybrids without chromosome 5 are as resistant as mouse cells.

Interferon: purification and initial characterization from human diploid cells.

  • E. Knight
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1976
Oxidation of this polypeptide with periodic acid and subsequent staining with Fuchsin base indicates that it contains carbohydrate ans suggests that the human fibroblast interferon is a glycoprotein.

Induction of Interferon in Chick Cells by Temperature-sensitive Mutants of Sindbis Virus

It is postulated that interferon induction is dependent on a low threshold level of RNA synthesis occurring early in infection as well as on the input multiplicity of the virus.

Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol

There is a marked effect of PEG concentration on cell hybridization, and there seem to be inherent differences between cells in terms of the extent of cell fusion induced by PEG.