Involvement of IS1 in the dissociation of the r-determinant and RTF components of the plasmid R100.1

@article{Chandler2004InvolvementOI,
  title={Involvement of IS1 in the dissociation of the r-determinant and RTF components of the plasmid R100.1},
  author={Mick Chandler and Bernard Allet and Edith Gallay and E. Boy de la Tour and Lucien G. Caro},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={153},
  pages={289-295}
}
SummaryThe formation of the r-determinant pLCl and of the RTF pAR132 from the composite plasmid R100.1 was investigated. The general location of IS1 sequences on the three plasmids was established by hybridization of λr14 CII::IS1 DNA to EcoRI generated fragments of the various plasmids separated by agarose gel electrophoresis and transferred directly to nitrocellulose filters. The position of IS1 sequences on these fragments and the homologies between fragments were analyzed by electron… Expand
Mapping of the drug resistance genes carried by the r-determinant of the R100.1 plasmid
TLDR
Cloning the EcoRI fragments of pLC1, a circular DNA element found in an Escherichia coli dnaAts strain integratively suppressed by R100.1 has allowed a more precise mapping of the position of the resistance genes on the R 100.1 molecule. Expand
Mapping of the resistance genes of the R plasmid NR1
SummaryThe drug resistance genes on the r-determinants component of the composite R plasmid NR1 were mapped on the EcoRI restriction endonuclease fragments of the R plasmid by cloning the fragmentsExpand
Some properties of the chloramphenicol resistance transposon Tn9
TLDR
Two distinct differences are demonstrated in the behavior of Tn9 but nor r-det, is able to amplify, by a recA dependent mechanism, when cells harboring RTF::Tn9 are grown in the presence of chloramphenicol, and Tn8 does not form extrachromosomal circular molecules when RTF ::Tn 9 is tegrated into the bacterial chromosome. Expand
On the role of IS1 in the formation of hybrids between the bacteriophage P1 and the R plasmid NR1
TLDR
The genomes of bacteriophage P1 derivatives carrying drug resistance genes derived from an R plasmid NR1 were analysed by restriction cleavage and be DNA-DNA hybridization to postulate that any genetic material carried adjacent to an IS1 element may undergo passive transposition. Expand
Production of extrachromosomal r-determinant circles from integrated R100.1: Involvement of the E. coli recombination system
TLDR
The results show that a specific replication of r-det sequences, not present in the wild-type R100.1 plasmid, occurs in these R-Hfr strains, and suggest that a rec promoted recombination, posterior to the specific replication event, is needed for the production of circular r-Det forms. Expand
The construction and replication properties of hybrid plasmids composed of the r-determinant of R100.1 and the plasmids pCRI or pSC201
TLDR
It is found that the hybrid plasmids segregate from cultures in which replication of the vector is blocked, suggesting that the r-det is not capable of autonomous replication. Expand
Sequence relationships between plasmids associated with conventional MLS resistance and zonal lincomycin resistance in Streptococcus pyogenes
TLDR
The results suggest that MLS and zonal lincomycin resistance have the same biochemical basis (i.e. methylation of 23S ribosomal RNA) and differ only quantitatively in the inducible control systems. Expand
Detection of free cytoplasmic circles of transposon Tn9 multimers in Escherichia coli
Extrachromosomal circular DNA molecules consisting of IS1-cat repeats, (IS1-cat)n, were isolated from an E. coli strain harboring nearly 30 copies of tandemly amplified transposon Tn9 located on theExpand
Complex Multiple Antibiotic and Mercury Resistance Region Derived from the r-det of NR1 (R100)
ABSTRACT The sequence of the 45.2-kb multidrug and mercury resistance region of pRMH760, a large plasmid from a clinical isolate of Klebsiella pneumoniae collected in 1997 in Australia, wasExpand
Tn10 mediated integration of the plasmid R100.1 into the bacterial chromosome: Inverse transposition
TLDR
It is suggested that integration of R100.1 was mediated by an inverse transposition using the IS10 sequences, and that at least 80% of the transposon segment located between them, and carrying the tetracycline resistance genes, had been lost. Expand
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