Involvement of GABAA receptors in the outgrowth of cultured hippocampal neurons

@article{Barbin1993InvolvementOG,
  title={Involvement of GABAA receptors in the outgrowth of cultured hippocampal neurons},
  author={G. Barbin and H. Pollard and J. Ga{\"i}arsa and Y. Ben‐Ari},
  journal={Neuroscience Letters},
  year={1993},
  volume={152},
  pages={150-154}
}
Whereas GABA is a major inhibitory neurotransmitter in the adult central nervous system, recent experiments performed in our laboratory have shown that the activation of GABAA receptors in the hippocampus leads to excitatory effects during the early post-natal period. The possible consequence of a depolarizing effect of GABA was assessed on the neuritic outgrowth of embryonic hippocampal neurons in culture. No morphological alterations were observed when hippocampal neurons were cultured for… Expand
GABAA receptor stimulation promotes survival of embryonic rat striatal neurons in culture.
TLDR
It is suggested that stimulation of GABAA receptors enhances survival of embryonic striatal neurons, and that the effect is mediated by Ca2+ influx through L-type voltage-dependent Ca 2+ channels, initiating intracellular signaling cascades that involve activation of H-7- and genistein-sensitive protein kinases. Expand
GABAA Receptors Mediate Trophic Effects of GABA on Embryonic Brainstem Monoamine Neurons In Vitro
TLDR
Results demonstrate that the activation/inhibition of GABAA receptors produces opposite effects on the development of embryonic monoamine and GABA neurons, which suggests that these neurotransmitter phenotypes may express GabAAreceptors that differ in fundamental ways, and these differences determine the developmental responses of these cells to GABAergic stimuli. Expand
Effects of GABA and GABA receptor inhibition on differentiation of mesencephalic precursors into dopaminergic neurons in vitro
TLDR
The results suggest that effects are exerted via GABA receptors located on DA precursors, and are not an indirect consequence of effects on the serotonergic or glial cell population. Expand
Postnatal maturation of gamma-aminobutyric acidA and B-mediated inhibition in the CA3 hippocampal region of the rat.
TLDR
It is reported that GABA mediates most of the excitatory drive at early stages of development in the hippocampal CA3 region and plays a trophic role in the neuritic outgrowth of cultured hippocampal neurons. Expand
Cholinergic cell expression in the developing rat medial septal nucleus in vitro is differentially controlled by GABAA and GABAB receptors
TLDR
It appears that endogenous GABA, tonically released in the developing septum, may, via specific receptor types, differentially control the biochemical maturation of the cholinergic neurons. Expand
Glutamate dependent dendritic outgrowth in developing neuronal networks of rat hippocampal cells in vitro
TLDR
It is suggested that glutamate release is crucial for the onset of dendritic morphological development in hippocampal neurons. Expand
Glutamate Inhibits GABA Excitatory Activity in Developing Neurons
TLDR
The data suggest that in developing neurons glutamate can inhibit the excitatory actions of GABA at both presynaptic and postsynaptic sites, and this may be one set of mechanisms whereby the actions of two excitatories transmitters, GABA and glutamate, do not lead to runaway excitation in the developing brain. Expand
GABAB Receptor Activation Triggers BDNF Release and Promotes the Maturation of GABAergic Synapses
TLDR
A novel mechanism by which endogenous GABA selectively regulates the development of GABAergic synapses in the developing brain is demonstrated by using whole-cell patch-clamp recordings and time-lapse fluorescence imaging on cultured hippocampal neurons expressing GFP-tagged brain-derived neurotrophic factor. Expand
GABAergic stimulation switches from enhancing to repressing BDNF expression in rat hippocampal neurons during maturation in vitro.
TLDR
Observations support the hypothesis that GABA might have neurotrophic effects on embryonic or perinatal hippocampal neurons, which are mediated by BDNF. Expand
GABA neurotransmission in the hypothalamus: developmental reversal from Ca2+ elevating to depressing
TLDR
It is suggested that GABA elevates Ca2+ in developing, but not mature, neurons from the hypothalamus and all other brain regions examined, or whether it might exist throughout the brain. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 28 REFERENCES
Involvement of gaba receptors in the regulation of neurite growth in cultured embryonic chick tectum
  • A. Michler
  • Medicine, Biology
  • International Journal of Developmental Neuroscience
  • 1990
TLDR
The data suggest that GABAA‐receptors may be involved in the GABA‐induced neurite elongation in serum‐supplemented medium only, although this subtype of receptors is present in serumfree conditions as well as revealed in binding studies using [3H]muscimol. Expand
Neurotrophic effects of GABA in cultures of embryonic chick brain and retina
TLDR
The hypothesis that GABA functions as a trophic or regulatory factor of at least certain neuron types of embryonic chick cortical and retinal neurons in culture is supported. Expand
Outgrowth-regulating actions of glutamate in isolated hippocampal pyramidal neurons
TLDR
Results indicate that activation of glutamate receptors leads to the opening of voltage-dependent calcium channels; the resulting increases in calcium influx lead to the observed alterations in dendritic outgrowth and neuronal survival. Expand
GABA influences the ultrastructure composition of cerebellar granule cells during development in culture
TLDR
The results strongly indicate that GABA in addition to being an important neurotransmitter serves as a trophic factor in the development of at least certain types of neurons. Expand
GABAergic neurons in rat hippocampal culture.
TLDR
The enrichment of GABAergic markers indicates that this preparation may be useful for the detailed study of hippocampal GABAergic neurons, when compared to the adult hippocampus in situ. Expand
The trophic effect of gaba on cerebellar granule cells is mediated by gaba-receptors
TLDR
It is found that while the density of mitochondria, the ribosomes and smooth endoplasmic reticulum was unaffected by this treatment the density in the cultured neurons was greatly enhanced by treatment of the cultured cells with GABA (50 μM) or THIP (150 μM). Expand
Neurotransmitters in the regulation of neuronal cytoarchitecture
  • M. Mattson
  • Biology, Medicine
  • Brain Research Reviews
  • 1988
TLDR
The neurotransmitter glutamate was found to specifically affect the cytoarchitecture of hippocampal pyramidal neuron dendrites in a graded manner which suggests that glutamate may be involved in: establishing hippocampal circuitry during brain development; maintaining and modifying circuitry in the adult; and inducing neurodegeneration in several disorders including epilepsy, Alzheimer's disease, and stroke. Expand
Distribution of GABAergic neurons in late fetal and early postnatal rat hippocampus.
TLDR
The results indicate that GABA may have a role in neurotransmission in the hippocampus from a very early stage of development and that GABA immunoreactivity appeared at the 18th day of gestation. Expand
GABAergic neurons of the hippocampus: Development in homotopic grafts and in dissociated cell cultures
TLDR
The hippocampus taken from E18-E19 rat embryos was dissociated into a cell suspension and was grafted into the hippocampus of adult rats or cultured, suggesting that GABA neurons express a high potential for growth and sprouting in vitro and in vivo. Expand
Use of central neuronal cultures for the detection of neuronotrophic agents
TLDR
Bioassay culture systems suitable for the identification of similar agents for intrinsic neurons of the central nervous system are established and conditioned media derived from rodent glial cell cultures, both primary and clonal, were able to support the survival of low density seeded central neurons. Expand
...
1
2
3
...