Involvement of FtsH in protein assembly into and through the membrane. I. Mutations that reduce retention efficiency of a cytoplasmic reporter.

To identify cellular factors that assist in membrane protein biogenesis, we looked for mutants affected in the "stop transfer" anchoring process. Using a SecY-PhoA fusion protein in which alkaline phosphatase (PhoA) mature sequence is attached to the last cytoplasmic domain following the 10th transmembrane segment of SecY, we isolated a mutation (std101… CONTINUE READING