Involvement of ADP-ribosylation factor 1 in cholera toxin-induced morphological changes of Chinese hamster ovary cells.

@article{Morinaga2001InvolvementOA,
  title={Involvement of ADP-ribosylation factor 1 in cholera toxin-induced morphological changes of Chinese hamster ovary cells.},
  author={Naoko Morinaga and Yuriko Kaihou and Nicol{\'a}s Marcelo Vitale and Joel Moss and Makoto Noda},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 25},
  pages={22838-43}
}
ADP-ribosylation factor 1 (ARF1) was originally found as a cofactor in CT-catalyzed ADP-ribosylation of Galpha(s) but is now known to participate in vesicle trafficking. We asked whether ARF1 function in vesicular trafficking is necessary for CT-induced morphological changes in Chinese hamster ovary (CHO) cells, which result from increased intracellular cAMP. Brefeldin A treatment of cells suppressed CT action, confirming a requirement for Golgi integrity. Overexpression of a GFP-ARF1 fusion… CONTINUE READING