Investigations of the F conjugation gene traI:traI mutants and lambdatraI transducing phages.

@article{Willetts1979InvestigationsOT,
  title={Investigations of the F conjugation gene traI:traI mutants and lambdatraI transducing phages.},
  author={N. G. Willetts and John Maule},
  journal={Molecular & general genetics : MGG},
  year={1979},
  volume={169 3},
  pages={325-36}
}
A series of traI point and deletion mutants of Flac, and a traM mutant, were characterised. Complementation tests with an amber Flac traI mutant confirmed their genotypes, and in addition all the traI mutants, but not the traM mutant, were complemented by pRS31 (PSC101 traDI) and EDlambda109 (lambdatraI). Judging from the efficiencies of plating of F-specific phages, none of the mutations affected pilus formation. The traI products of F and of the F-like plasmid R1 were interchangeable with… CONTINUE READING

Similar Papers

Loading similar papers…