Investigation on the mechanism by which fructose, hexitols and other compounds regulate the translocation of glucokinase in rat hepatocytes.

@article{Niculescu1997InvestigationOT,
  title={Investigation on the mechanism by which fructose, hexitols and other compounds regulate the translocation of glucokinase in rat hepatocytes.},
  author={Liliana Daniela Niculescu and Maria Veiga-da-Cunha and Emile Van Schaftingen},
  journal={The Biochemical journal},
  year={1997},
  volume={321 ( Pt 1)},
  pages={
          239-46
        }
}
In isolated hepatocytes in suspension, the effect of sorbitol but not that of fructose to increase the concentration of fructose 1-phosphate and to stimulate glucokinase was abolished by 2-hydroxymethyl-4-(4-N,N-dimethylamino-1-piperazino)-pyrimidine (SDI 158), an inhibitor of sorbitol dehydrogenase. In hepatocytes in primary culture, fructose was metabolized at approximately one-quarter of the rate of sorbitol, and was therefore much less potent than the polyol in increasing the concentration… Expand
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