Investigation of the substrate specificity of a beta-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis.

@article{Marana2004InvestigationOT,
  title={Investigation of the substrate specificity of a beta-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis.},
  author={Sandro R Marana and Eduardo H P Andrade and Cl{\'e}lia Ferreira and Walter R. Terra},
  journal={European journal of biochemistry},
  year={2004},
  volume={271 21},
  pages={4169-77}
}
The specificity of the Spodoptera frugiperda digestive beta-glycosidase (Sfbetagly50) for fucosides, glucosides and galactosides is determined by noncovalent interactions of glycone 6-OH and glycone 4-OH with the active-site residues Q39 and E451. Site-directed mutagenesis and enzyme steady-state kinetics were described, showing that replacement of E451 with glutamine increased the preference of Sfbetagly50 for glucosides in comparison to galactosides, whereas replacing E451 with serine had the… CONTINUE READING