Investigation of the role of tyrosine-114 in the activity of human O6-alkylguanine-DNA alkyltranferase.

@article{Goodtzova1998InvestigationOT,
  title={Investigation of the role of tyrosine-114 in the activity of human O6-alkylguanine-DNA alkyltranferase.},
  author={Karma Goodtzova and Sreenivas Kanugula and Suvarchala Edara and Anthony E. Pegg},
  journal={Biochemistry},
  year={1998},
  volume={37 36},
  pages={
          12489-95
        }
}
Tyrosine-114 is one of 13 totally conserved amino acids in all known sequences of O6-alkylguanine-DNA alkyltransferase (AGT). The importance of this amino acid in repair of alkylated DNA by AGT was studied by changing it to phenylalanine (F), alanine (A), threonine (T), or glutamic acid (E) in human AGT. The activities of the mutant proteins were then compared to those of the wild type with regard to abilities to do the following: (a) protect Escherichia coli from the methylating agent N-methyl… CONTINUE READING
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