Investigation of the role of calpain as a stimulus-response mediator in human platelets using new synthetic inhibitors.

@article{Anagli1991InvestigationOT,
  title={Investigation of the role of calpain as a stimulus-response mediator in human platelets using new synthetic inhibitors.},
  author={J Anagli and J{\"o}rg Hagmann and Elwood R. Shaw},
  journal={The Biochemical journal},
  year={1991},
  volume={274 ( Pt 2)},
  pages={497-502}
}
A series of peptidyl diazomethanes and monofluoromethane with structures specific for calpain have been synthesized and tested for their ability to inhibit calpain activity in vivo, using human platelets as a model system. Calpain activity in vivo was determined by observing proteolysis of actin-binding protein and talin, two known substrates of calpain. Very potent inhibitors, which emerged from this study, were used to investigate the role of calpain in some platelet response processes. Our… CONTINUE READING