Investigation of the correlation between charge and glycosylation of IgG1 variants by liquid chromatography-mass spectrometry.

@article{Yang2014InvestigationOT,
  title={Investigation of the correlation between charge and glycosylation of IgG1 variants by liquid chromatography-mass spectrometry.},
  author={Jia-Ming Yang and Junwen Ai and Yuemei Bao and Zhijun Yuan and Yumin Qin and Yi-wu Xie and Desheng Tao and Daotian Fu and Yucai Peng},
  journal={Analytical biochemistry},
  year={2014},
  volume={448},
  pages={82-91}
}
A recombinant IgG1 monoclonal antibody (mAb) showed multiple charge variants in a cation exchange chromatography profile. To better understand the correlation between charge heterogeneity and glycosylation, a rapid reversed phase ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method with integrated mass analysis has been developed for simultaneous determination of N-terminal pyroglutamate, C-terminal lysine truncation, and Fc glycosylation. The results show that various… CONTINUE READING

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