Investigation of the Cytotoxicity of Antimicrobial Peptide P40 on Eukaryotic Cells

  title={Investigation of the Cytotoxicity of Antimicrobial Peptide P40 on Eukaryotic Cells},
  author={Rodrigo de Almeida Vaucher and M{\'a}rio Lettieri Teixeira and Adriano Brandelli},
  journal={Current Microbiology},
The in vitro cytotoxicity of the antimicrobial peptide P40 was investigated. The food grade bacteriocin nisin was also analyzed for comparison. VERO cells were treated with different concentrations (0.02–2.5 μg ml−1) of nisin and P40, and cell viability and plasma membrane integrity were checked by MTT, neutral red uptake (NRU), and lactate dehydrogenase (LDH) assays. In MTT and NRU assays the EC50 to the purified peptide P40 were 0.30 and 0.51 μg ml−1, while values found to nisin were 0.35 and… CONTINUE READING


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