Investigation of pregnancy induced adrenergic denervation in the rat PhD Thesis


The pregnancy-induced rapid degeneration of the adrenergic nerves innervating the uterus is a well-known but poorly under-<lb>stood phenomenon. Since most of the published investigations were carried out by histological assay, our aim was to describe<lb>the loss of the adrenergic function during pregnancy and the reinnervational procedure in the postpartum period. Myometrial<lb>and cervical samples from rats were loaded with<lb>[H]noradrenaline and then transferred into a chamber for superfusion. After<lb>a wash-out period, fractions were collected. The fifth and fifteenth fraction tissues were stimulated with an electric field. The<lb>[H]noradrenaline contents of the fractions were determined, together with the amount remaining in the tissue. The myome-<lb>trial [H]noradrenaline release was substantially decreased in early pregnancy, and absent in the late stage. Differences in<lb>release profile were detected between the implantation sites and the interimplantation areas. As a refinement of the results of<lb>previous histochemical studies, the noradrenergic functions of the cervix were found to be deeply affected in the early post-<lb>partum period. The pregnancy-induced denervational procedure can be followed by means of a superfusional technique after<lb>[H]noradrenaline loading. As our technique is considered to be similar in sensitivity to histological methods, superfusion can<lb>be regarded as a model for functional investigations of pregnancy-induced denervation.<lb>Reproduction (2005) 130 743–749

19 Figures and Tables

Cite this paper

@inproceedings{Csonka2010InvestigationOP, title={Investigation of pregnancy induced adrenergic denervation in the rat PhD Thesis}, author={D{\'e}nes Csonka}, year={2010} }