Investigation of opioid receptor binding protein in adult and fetal mouse brain membranes by photoaffinity labeling.


125I-[D-Ala2,p-N3-Phe4-Met5]Enkephalin was used to investigate the opioid receptor from brain membranes of adult (6 months old) and fetal (16 days gestation) mice. The photolabeled membranes were analyzed by sodium dodecyl sulfate gel electrophoresis. A 46,000-Da protein was specifically photo-labeled in the adult mouse brain membranes. The photolabeling of… (More)