Investigation of limiting metabolic steps in the utilization of xylose by recombinant Saccharomyces cerevisiae using metabolic engineering.

@article{Karhumaa2005InvestigationOL,
  title={Investigation of limiting metabolic steps in the utilization of xylose by recombinant Saccharomyces cerevisiae using metabolic engineering.},
  author={Kaisa Karhumaa and B{\"a}rbel Hahn-H{\"a}gerdal and Marie F. Gorwa-Grauslund},
  journal={Yeast},
  year={2005},
  volume={22 5},
  pages={359-68}
}
A Saccharomyces cerevisiae screening strain was designed by combining multiple genetic modifications known to improve xylose utilization with the primary objective of enhancing xylose growth and fermentation in xylose isomerase (XI)-expressing strains. Strain TMB 3045 was obtained by expressing the XI gene from Thermus thermophilus in a strain in which the GRE3 gene coding for aldose reductase was deleted, and the genes encoding xylulokinase (XK) and the enzymes of the non-oxidative pentose… CONTINUE READING
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