Investigation of epoxide hydrolase activity in Saccharomyces cerevisiae ORF YNR064c protein

Abstract

The histidine-tagged variant of YNR064c protein was heterologously expressed in competent E. coli BL21 AI cells and the protein was successfully purified with immobilized metal ion affinity chromatography. The protein YNR064c displayed low epoxide hydrolase activity in a single measurement with 0.2 mM (S)-2phenyloxirane but the activity was not reproducible… (More)

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