Investigation of enzymatic C–P bond formation using multiple quantum HCP nuclear magnetic resonance spectroscopy

  title={Investigation of enzymatic C–P bond formation using multiple quantum HCP nuclear magnetic resonance spectroscopy},
  author={K. Hu and W. J. Werner and Kylie D. Allen and Susan C Wang},
  journal={Magnetic Resonance in Chemistry},
  pages={267 - 272}
The biochemical mechanism for the formation of the C–P–C bond sequence found in l‐phosphinothricin, a natural product with antibiotic and herbicidal activity, remains unclear. To obtain further insight into the catalytic mechanism of PhpK, the P‐methyltransferase responsible for the formation of the second C–P bond in l‐phosphinothricin, we utilized a combination of stable isotopes and two‐dimensional nuclear magnetic resonance spectroscopy. Exploiting the newly emerged Bruker QCI probe (Bruker… Expand
Mechanistic studies on CysS - a Vitamin B12-dependent radical SAM methyltransferase involved in the biosynthesis of the t-butyl group of cystobactamid.
  • Yuanyou Wang, T. Begley
  • Medicine, Chemistry
  • Journal of the American Chemical Society
  • 2020
Three experiments are described to elucidate how CysS uses Cbl, SAM, and a [4Fe- 4S] cluster to catalyze iterative methylation reactions: a cyclopropylcarbinyl rearrangement was used to trap the substrate radical and to estimate the rate of the radical substitution reaction involved in the methyl transfer. Expand
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  • Chemistry, Medicine
  • Current opinion in chemical biology
  • 2009
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In Vitro Characterization of a Heterologously Expressed Nonribosomal Peptide Synthetase Involved in Phosphinothricin Tripeptide Biosynthesis†
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