Investigation of enzymatic C–P bond formation using multiple quantum HCP nuclear magnetic resonance spectroscopy

@article{Hu2015InvestigationOE,
  title={Investigation of enzymatic C–P bond formation using multiple quantum HCP nuclear magnetic resonance spectroscopy},
  author={K. Hu and W. J. Werner and Kylie D. Allen and Susan C Wang},
  journal={Magnetic Resonance in Chemistry},
  year={2015},
  volume={53},
  pages={267 - 272}
}
The biochemical mechanism for the formation of the C–P–C bond sequence found in l‐phosphinothricin, a natural product with antibiotic and herbicidal activity, remains unclear. To obtain further insight into the catalytic mechanism of PhpK, the P‐methyltransferase responsible for the formation of the second C–P bond in l‐phosphinothricin, we utilized a combination of stable isotopes and two‐dimensional nuclear magnetic resonance spectroscopy. Exploiting the newly emerged Bruker QCI probe (Bruker… Expand
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