Investigation of cationic peanut peroxidase glycans by electrospray ionization mass spectrometry.

@article{Zhang2004InvestigationOC,
  title={Investigation of cationic peanut peroxidase glycans by electrospray ionization mass spectrometry.},
  author={Cunjie Zhang and Amanda Doherty-Kirby and Robert van Huystee Rv and Gilles A. Lajoie},
  journal={Phytochemistry},
  year={2004},
  volume={65 11},
  pages={
          1575-88
        }
}
Cationic peanut peroxidase (CP) was isolated from peanut (Arachis hypogaea) cell suspension culture medium. CP is a glycoprotein with three N-linked glycan sites at Asn60, Asn144, and Asn185. ESI-MS of the intact purified protein reveals the microheterogeneity of the glycans. Tryptic digestion of CP gave a near complete sequence coverage by ESI-MS. The glycopeptides from the tryptic digestion were separated by RP HPLC identified by ESI-MS and the structure of the glycan chains determined by ESI… CONTINUE READING

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