Investigation of adenosine base ionization in the hairpin ribozyme by nucleotide analog interference mapping.

@article{Ryder2001InvestigationOA,
  title={Investigation of adenosine base ionization in the hairpin ribozyme by nucleotide analog interference mapping.},
  author={Sean P. Ryder and Adegboyega K Oyelere and Jose Luis Padilla and Dagmar Klostermeier and David P. Millar and Scott A Strobel},
  journal={RNA},
  year={2001},
  volume={7 10},
  pages={1454-63}
}
Tertiary structure in globular RNA folds can create local environments that lead to pKa perturbation of specific nucleotide functional groups. To assess the prevalence of functionally relevant adenosine-specific pKa perturbation in RNA structure, we have altered the nucleotide analog interference mapping (NAIM) approach to include a series of a phosphorothioate-tagged adenosine analogs with shifted N1 pKa values. We have used these analogs to analyze the hairpin ribozyme, a small self-cleaving… CONTINUE READING
18 Citations
36 References
Similar Papers

References

Publications referenced by this paper.
Showing 1-10 of 36 references

Ferre-D’Amare AR+ 2001+ Crystal structure of a hairpin ribozyme-inhibitor complex with implications for catalysis+

  • PB Rupert
  • Nature
  • 2001
Highly Influential
5 Excerpts

+ Crystal structure of a hairpin ribozymeinhibitor complex with implications for catalysis +

  • SP Ryder, L Ortoleva-Donnelly, AB Kosek, SA Strobel
  • Nature
  • 2001

Biochemical detection of cytidine protonation within RNA+

  • AK Oyelere, SA Strobel
  • J Am Chem
  • 2000
2 Excerpts

+ Structural basis for the guanosine requirement of the hairpin ribozyme +

  • FM Richards, HW Wyckoff, WD Carlson, NM Allewell, B Lee, Y Mitsui
  • Biochemistry
  • 1999

Similar Papers

Loading similar papers…