Investigation of a genetically engineered mutant of barnacle troponin C containing a central helix deletion


To examine the importance of the central α-helix of troponin C (TnC) we have bacterially expressed one of the isoforms of barnacle TnC (BTnC2), BTnCWT, but without the aspartate residue at position 80 in the central helix (BTnC80–). This manipulation is expected to produce an approximately 100° axial rotation of the C-domain with respect to the N-domain… (More)
DOI: 10.1007/s004249900133


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