Investigation of DNA methylation by direct electrocatalytic oxidation.

@article{Wang2010InvestigationOD,
  title={Investigation of DNA methylation by direct electrocatalytic oxidation.},
  author={Po Wang and Zhibin Mai and Zong Dai and Xiaoyong Zou},
  journal={Chemical communications},
  year={2010},
  volume={46 41},
  pages={
          7781-3
        }
}
A rapid, convenient and accurate method for the assessment of DNA methylation status is presented based on the direct electrocatalytic oxidation of DNA bases without any bisulfite conversion, PCR amplification or labeling procedures. 
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52 Citations
Highly Influential Citations
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Background Citations
6
Methods Citations
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52 Citations

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Citation Type

An electrochemical approach for detection of DNA methylation and assay of the methyltransferase activity.
TLDR
An electrochemical approach for rapid detection of the genomic DNA methylation level, assay of methyltransferase activity, and evaluation and screening of the inhibitors of methyl transferase may be a help for the discovery of anticancer drugs.
A label-free electrochemical assay for quantification of gene-specific methylation in a nucleic acid sequence.
A label-free electrochemical method is developed for simple and convenient quantification of gene-specific DNA hypermethylation in a DNA sequence without PCR amplification, bisulfite conversion or…
Simultaneous profiling of multiple gene-methylation loci by electrochemical methylation-specific ligase detection reaction.
A novel method of electrochemical methylation-specific ligation detection reaction is first presented for simultaneous evaluation of multiple gene-methylation loci in a single-tube experiment without…
Detection of DNA base variation and cytosine methylation at a single nucleotide site using a highly sensitive fluorescent probe.
A fluorescent DNA probe containing an anthracene group attached via an anucleosidic linker can identify all four DNA bases at a single site as well as the epigenetic modification C/5-MeC via a…
Electrochemical evaluation of DNA methylation level based on the stoichiometric relationship between purine and pyrimidine bases.
Picomolar level profiling of the methylation status of the p53 tumor suppressor gene by a label-free electrochemical biosensor.
An efficient electrochemical approach is developed for ultrasensitive profiling of the methylation status of the p53 tumor suppressor gene based on a label-free biosensor in combination with…
Gold nanorods-based FRET assay for ultrasensitive detection of DNA methylation and DNA methyltransferase activity.
TLDR
It is demonstrated that this method is capable of detecting methyltransferase with a detection limit of 0.25 U mL(-1), which might make this method a good candidate for monitoring DNA methylation in the future.
A label-free supersandwich electrogenerated chemiluminescence method for the detection of DNA methylation and assay of the methyltransferase activity.
TLDR
It is demonstrated that the label-free ECL method is capable of detecting methyltransferase with a detection limit of 3 × 10(-6) U mL(-1), using a supersandwich amplification technique.
Electrochemical immunosensing platform for DNA methyltransferase activity analysis and inhibitor screening.
TLDR
A novel electrochemical method to quantify DNA methyltransferase (MTase) and analyze DNA MTase activity and inhibition investigation demonstrated that, in the presence of 160 μM S-adenosyl-L-methionine as methyl donor, 5-aza-2'-deoxycytidine, procaine, epicatechin, and caffeic acid could inhibit the M. SssIMTase activity.
Electrochemical biosensing method for the detection of DNA methylation and assay of the methyltransferase activity
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