Investigation of DNA extraction from hair shafts

  title={Investigation of DNA extraction from hair shafts},
  author={Kazuho Takayanagi and Hideki Asamura and Keiji Tsukada and Moriyoshi Ota and Shin Saito and Hiromichi Fukushima},
Human hair shafts can be important forensic evidence for identification, but DNA typing, even of mitochondrial DNA (mtDNA), presented certain difficulties. We describe three DNA extraction methods from hair shafts, such as the phenol/chloroform method, NaI treatment method, and silicabeads method. In order to make an investigation of mtDNA amplification rate and efficiency, the amplifications of the mtDNA control region (D-loop) HV1A (15997–16262) used FAM-labeled forward primer. As a result of… CONTINUE READING
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