Investigation of Chromosomes in Varieties and Translocation Lines of Pea Pisum sativum L. by FISH, Ag-NOR, and Differential DAPI Staining

@article{Samatadze2005InvestigationOC,
  title={Investigation of Chromosomes in Varieties and Translocation Lines of Pea Pisum sativum L. by FISH, Ag-NOR, and Differential DAPI Staining},
  author={Tatiana E. Samatadze and Olga V Muravenko and Nadezhda L Bolsheva and Alexandra V Amosova and Sergei Gostimsky and Alexandr V. Zelenin},
  journal={Russian Journal of Genetics},
  year={2005},
  volume={41},
  pages={1381-1388}
}
The DNA intercalator 9-aminoachridine was used for obtaining high-resolution DAPI patterns of chromosomes of Pisum sativum L. with more than 300 bands per haploid chromosome set. The karyotypes of three pea varieties, Viola, Capital, and Rosa Crown, and two translocation lines, L-108 (T2–4s) and M-10 (T2–7s), were examined. Based on the results of DAPI staining, we have identified chromosomes, constructed idiograms, and established breakpoints of chromosome translocations. Lines L-108 (T2–4s… CONTINUE READING