Investigation of 6-thiodeoxyguanosine alkylation products and their role in the potentiation of BCNU cytotoxicity.


The principal products of the reaction of 6-thio-2'-deoxyguanosine (6-TdGuo) with dimethylsulfate are S6-methyl-6-thiodeoxyguanosine and 7-methyl-6-thiodeoxyguanosine, identified by ultraviolet and mass spectrometry. To study the reactions of 6-TdGuo in DNA, cells were treated with 6-thioguanine, which is incorporated into DNA during S-phase; DNA was… (More)