Investigating the specific interactions between carbonic anhydrase and a sulfonamide inhibitor by single-molecule force spectroscopy.

@article{Kamper2007InvestigatingTS,
  title={Investigating the specific interactions between carbonic anhydrase and a sulfonamide inhibitor by single-molecule force spectroscopy.},
  author={Sarah G Kamper and Laura Porter-Peden and Ronald L Blankespoor and Kumar Sinniah and Dejian Zhou and Chris Abell and Trevor Rayment},
  journal={Langmuir : the ACS journal of surfaces and colloids},
  year={2007},
  volume={23 25},
  pages={12561-5}
}
In this communication, we report on the interaction landscape of an active site-specific enzyme-inhibitor complex by single-molecule force spectroscopy. Electrostatic immobilization was employed to orient a carbonic anhydrase enzyme on a positively charged surface so its active site is pointing upward. This approach to immobilization effectively increases… CONTINUE READING