Investigating the role of H₂S in 4-HNE scavenging.

Abstract

4-HNE (4-hydroxy-2-nonenal) is a highly reactive α,β-unsaturated aldehyde generated from oxidation of polyunsaturated fatty acids and has been suggested to play a role in the pathogenesis of several diseases. 4-HNE can bind to amino acids, proteins, polynucleotides, and lipids and exert cytotoxicity. 4-HNE forms adducts (Michael adducts) with cysteine, lysine, as well as histidine on proteins with the thiol function as the most reactive nucleophilic moiety. Thus, detoxification strategies by 4-HNE scavenging compounds might be of interest. Recently, hydrogen sulfide (H2S) has been identified as an endogenous vascular gasotransmitter and neuromodulator. Assuming that the low-molecular thiol H2S may react with 4-HNE, methods to monitor the ability of H2S to counteract the protein-modifying and cytotoxic activity of 4-HNE are described in this chapter.

DOI: 10.1016/bs.mie.2014.11.018

Cite this paper

@article{Laggner2015InvestigatingTR, title={Investigating the role of H₂S in 4-HNE scavenging.}, author={Hilde Laggner and Bernhard M. K. Gmeiner}, journal={Methods in enzymology}, year={2015}, volume={555}, pages={3-18} }