Investigating the macropinocytic proteome of Dictyostelium amoebae by high-resolution mass spectrometry.

@article{Journet2012InvestigatingTM,
  title={Investigating the macropinocytic proteome of Dictyostelium amoebae by high-resolution mass spectrometry.},
  author={Agn{\`e}s Journet and G{\'e}rard Klein and Sabine Brugi{\`e}re and Yves Vandenbrouck and Agn{\`e}s Chapel and Sylvie Kieffer and Christophe Bruley and Christophe D Masselon and Laurence Aubry},
  journal={Proteomics},
  year={2012},
  volume={12 2},
  pages={241-5}
}
The cellular slime mold Dictyostelium discoideum is a soil-living eukaryote, which feeds on microorganisms engulfed by phagocytosis. Axenic laboratory strains have been produced that are able to use liquid growth medium internalized by macropinocytosis as the source of food. To better define the macropinocytosis process, we established the inventory of proteins associated with this pathway using mass spectrometry-based proteomics. Using a magnetic purification procedure and high-performance LC… CONTINUE READING
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