Investigating the Role of Adducts in Protein Supercharging with Sulfolane

@article{Douglass2012InvestigatingTR,
  title={Investigating the Role of Adducts in Protein Supercharging with Sulfolane},
  author={Kevin Aart Douglass and Andre R Venter},
  journal={Journal of The American Society for Mass Spectrometry},
  year={2012},
  volume={23},
  pages={489-497}
}
The supercharging effect of sulfolane on cytochrome c (cyt c) during electrospray ionization mass spectrometry (ESI-MS) in the absence of conformational effects was investigated. The addition of sulfolane on the order of 1 mM or greater to denaturing solutions of cyt c results in supercharging independent of protein concentration over the range of 0.1 to 10 μM. While supercharging was observed in the positive mode, no change in the charge state distribution was observed in the negative mode… CONTINUE READING

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