Investigating microbial eukaryotic diversity from a global census: insights from a comparison of pyrotag and full-length sequences of 18S rRNA genes.

@article{Lie2014InvestigatingME,
  title={Investigating microbial eukaryotic diversity from a global census: insights from a comparison of pyrotag and full-length sequences of 18S rRNA genes.},
  author={Alle A Y Lie and Zhenfeng Liu and Sarah K Hu and Adriane Clark Jones and Diane Y. Kim and Peter D. Countway and Linda A Amaral-Zettler and Stephen C. Cary and Evelyn B. Sherr and Barry F. Sherr and Rebecca J. Gast and David A. Caron},
  journal={Applied and environmental microbiology},
  year={2014},
  volume={80 14},
  pages={4363-73}
}
Next-generation DNA sequencing (NGS) approaches are rapidly surpassing Sanger sequencing for characterizing the diversity of natural microbial communities. Despite this rapid transition, few comparisons exist between Sanger sequences and the generally much shorter reads of NGS. Operational taxonomic units (OTUs) derived from full-length (Sanger sequencing) and pyrotag (454 sequencing of the V9 hypervariable region) sequences of 18S rRNA genes from 10 global samples were analyzed in order to… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 20 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 51 references

Regional variability in eukaryotic protist communities in the Amundsen Sea

C Wolf, S Frickenhaus, ES Kilias, I Peeken, K. Metfies
Antarctic Sci • 2013
View 1 Excerpt

Similar Papers

Loading similar papers…